Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Adropin antibody [EPR24382-57] - BSA and Azide free (ab279721)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24382-57] to Adropin - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Adropin antibody [EPR24382-57] - BSA and Azide free
    See all Adropin primary antibodies

  • Description

    Rabbit monoclonal [EPR24382-57] to Adropin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt (Intra),ICC,IHC-Fr or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain and cerebellum; Rat brain; Human cerebellum tissue lysates; Human Adropin Recombinant Protein (aa38-79) with a His6-ABP tag. IP: Mouse brain; Rat brain tissue lysates.

  • General notes

    ab279721 is the carrier-free version of ab279716. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab279721 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Adropin antibody [EPR24382-57] - BSA and Azide free (ab279721)
    Western blot - Anti-Adropin antibody [EPR24382-57] - BSA and Azide free (ab279721)
    Anti-Adropin antibody [EPR24382-57] (ab279716) at 1/1000 dilution + Human Adropin Recombinant Protein (aa38-79) with a His6-ABP tag at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 8 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab279716, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     Exposure time: 8 seconds

     

  • Western blot - Anti-Adropin antibody [EPR24382-57] - BSA and Azide free (ab279721)
    Western blot - Anti-Adropin antibody [EPR24382-57] - BSA and Azide free (ab279721)
    Anti-Adropin antibody [EPR24382-57] (ab279716) at 1/1000 dilution + Human cerebellum tissue lysate at 20 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 8 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?



    This data was developed using ab279716, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes

     

  • Western blot - Anti-Adropin antibody [EPR24382-57] - BSA and Azide free (ab279721)
    Western blot - Anti-Adropin antibody [EPR24382-57] - BSA and Azide free (ab279721)
    All lanes : Anti-Adropin antibody [EPR24382-57] (ab279716) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse cerebellum tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse lung tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 8 kDa
    Observed band size: 15,17 kDa why is the actual band size different from the predicted?



    This data was developed using ab279716, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression tissues: lung, kidney (PMID:25074942).

    The expression profile / molecular weight  observed is consistent with what has been described in the literature (PMID:25074942).

    Exposure time: 3 minutes

     

  • Immunoprecipitation - Anti-Adropin antibody - BSA and Azide free (ab279721)
    Immunoprecipitation - Anti-Adropin antibody - BSA and Azide free (ab279721)

    This data was developed using ab279716, the same antibody clone in a different buffer formulation.

    Adropin was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab279716 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab279716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: ab279716 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab279716 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 6 seconds

  • Immunoprecipitation - Anti-Adropin antibody - BSA and Azide free (ab279721)
    Immunoprecipitation - Anti-Adropin antibody - BSA and Azide free (ab279721)

    This data was developed using ab279716, the same antibody clone in a different buffer formulation.

    Adropin was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab279716 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab279716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10 ug

    Lane 2: ab279716 IP in Rat brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab279716 in rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds

     

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