Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free (ab238782)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP226] to Caldesmon/CDM - BSA and Azide free
Suitable for: WB, IHC-P, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free
See all Caldesmon/CDM primary antibodies
Description

Rabbit monoclonal [SP226] to Caldesmon/CDM - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cytmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human colon tissue; Mouse colon tissue; Rat colon tissue Flow Cyt: HeLa cells. WB: HeLa and NIH/3T3 cell lysates.
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
ab238782 is the carrier-free version of ab183339 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab238782 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Chicken: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Caldesmon
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP226
Isotype

IgG
Research areas

Western blot - Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free (ab238782)

All lanes : Anti-Caldesmon/CDM antibody [SP226] (ab183339) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CALD1 knockout HeLa cell lysate
Lane 3 : NIH/3T3 cell lysate
Lane 4 : HEK-293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 93 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

This data was developed using the same antibody clone in a different buffer formulation (ab183339).

Lanes 1-4: Merged signal (red and green). Green - ab183339 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.

ab183339 Anti-Caldesmon/CDM antibody [SP226] was shown to specifically react with Caldesmon/CDM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265026 (knockout cell lysate ab257375) was used. Wild-type and Caldesmon/CDM knockout samples were subjected to SDS-PAGE. ab183339 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free (ab238782)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling Caldesmon/CDM with ab183339 at 1/100 dilution (23.11 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on smooth muscle in rat colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183339 for 10 mins at room temperature. This image was generated using ab183339, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free (ab238782)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling Caldesmon/CDM with ab183339 at 1/100 dilution (23.11 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on smooth muscle in mouse colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183339 for 10 mins at room temperature. This image was generated using ab183339, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free (ab238782)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Caldesmon/CDM with ab183339 at 1/100 dilution (23.11 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on smooth muscle in human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183339 for 10 mins at room temperature. This image was generated using ab183339, the same clone, but with a different buffer formulation.
Flow Cytometry - Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free (ab238782)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) labeling Caldesmon/CDM with purified ab183339 at 1/230 dilution (10.05 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183339).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free (ab238782)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon tissue labeling Caldesmon/CDM with ab183339 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183339).
Flow Cytometry - Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free (ab238782)

Flow cytometric analysis of HeLa cells labeling Caldesmon/CDM with ab183339 at 1/400 dilution (green) compared to a negative control Rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183339).
Anti-Caldesmon/CDM antibody [SP226] - BSA and Azide free (ab238782)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com