Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Dystrophin antibody [EPR23336-129]
See all Dystrophin primary antibodies
Description

Rabbit monoclonal [EPR23336-129] to Dystrophin
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human skeletal muscle tissue lysate; Mouse skeletal muscle and heart tissue lysates; Rat skeletal muscle tissue lysate. IHC-P: Human skeletal muscle and cardiac muscle tissue; Mouse skeletal muscle and cardiac muscle tissue; Rat skeletal muscle and cardiac muscle tissue. IHC-Fr: Mouse retina tissue; Rat retina tissue. IP: Mouse skeletal muscle tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23336-129
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] (ab275391)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on mouse skeletal muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX. instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Dystrophin antibody [EPR23336-129] (ab275391)

All lanes : Anti-Dystrophin antibody [EPR23336-129] (ab275391) at 1/1000 dilution

Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Rat skeletal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

Predicted band size: 427 kDa
Observed band size: 260,427 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

427-kDa and 260 kDa Dystrophin are observed. The molecular weight observed is consistent with what have been described in literature (PMID: 7633443, 9224291, 10675908).

Other weak immunoreactive bands may indicate degradation products (PMID: 9224291).

Exposure time: 48 seconds.
Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR23336-129] (ab275391)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse retina tissue labeling Dystrophin with ab275391 at 1/100 (5.61 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488) at 1/1000 dilution (Green). Positive staining on mouse retina. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunoprecipitation - Anti-Dystrophin antibody [EPR23336-129] (ab275391)

Dystrophin was immunoprecipitated from 0.35 mg mouse skeletal muscle tissue lysate with ab275391 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275391 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse skeletal muscle tissue lysate 10 ug

Lane 2: ab275391 IP in Mouse skeletal muscle tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275391 in mouse skeletal muscle tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 76 seconds

427-kDa and 260 kDa Dystrophin are observed. The molecular weight observed is consistent with what have been described in literature (PMID: 7633443, 9224291, 10675908). Other weak immunoreactive bands may indicate degradation products(PMID: 9224291).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] (ab275391)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on human skeletal muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] (ab275391)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on rat cardiac muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR23336-129] (ab275391)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat retina tissue labeling Dystrophin with ab275391 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat retina. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] (ab275391)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on rat skeletal muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] (ab275391)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cardiac muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] (ab275391)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on human cardiac muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-Dystrophin antibody [EPR23336-129] (ab275391)