Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11410-43] to Bcl6 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free
    See all Bcl6 primary antibodies

  • Description

    Rabbit monoclonal [EPR11410-43] to Bcl6 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    We tested the antibody in mouse and rat samples and tests were unsuccessful therefore we can no longer guaranteed the reactivity of this species.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human bcl-6 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P41182

  • General notes

    Ab249707 is the carrier-free version of ab172610. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249707 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)

    This data was developed using ab172610, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node in stomach tissue sections labeling bcl-6 with purified ab172610 at 1:1500 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
    Western blot - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
    Anti-Bcl6 antibody [EPR11410-43] (ab172610) at 1/2000 dilution (purified) + Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg

    Predicted band size: 78 kDa
    Observed band size: 78 kDa



    This data was developed using ab172610, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)

    This data was developed using ab172610, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
  • Western blot - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
    Western blot - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
    Anti-Bcl6 antibody [EPR11410-43] (ab172610) at 1/1000 dilution + Ramos cell lysate at 10 µg

    Predicted band size: 78 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
    This data was developed using ab172610, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
    This data was developed using ab172610, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human B cell lymphoma tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
  • Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)
    Anti-Bcl6 antibody [EPR11410-43] - BSA and Azide free (ab249707)

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