Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (ab274326)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20925-114] to TIFA - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-TIFA antibody [EPR20925-114] - BSA and Azide free
    See all TIFA primary antibodies

  • Description

    Rabbit monoclonal [EPR20925-114] to TIFA - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T and MOLT-4 whole cell lysates.

  • General notes

    ab274326 is the carrier-free version of ab239352. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab274326 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (ab274326)
    Western blot - Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (ab274326)
    All lanes : Anti-TIFA antibody [EPR20925-114] (ab239352) at 1/1000 dilution

    Lane 1 : TIFA knockout HEK-93T whole cell lysate
    Lane 2 : Wild-type HEK-293T whole cell lysate
    Lane 3 : MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

    Predicted band size: 21 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab239352, the same antibody clone in a different buffer formulation. 

    Data was kindly provided by collaborator.

    ab239352 Anti-TIFA antibody [EPR20925-114] was shown to react with TIFA in HEK-293T cells in Western blot. Loss of signal was observed when TIFA knockout sample was used. Wild-type and TIFA knockout samples were subjected to SDS-PAGE. ab239352 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging.  Anti-beta Tubulin was used as loading control.

    Exposure time: 2 minutes.

     

  • Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (ab274326)
    Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (ab274326)

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