Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5955(2)] to TTF1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free
    See all TTF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5955(2)] to TTF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Human fetal lung, Rat lung and Mouse lung lysates; Human lung adenocarcinoma and Human papillary carcinoma of thyroid gland tissues.

  • General notes

    Ab227574 is the carrier-free version of ab133737. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab227574 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 9.50 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5955(2)

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)

    Immunohistochemical analysis using ab133737 showing negative staining in Normal tonsil tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)

    Immunohistochemical analysis using ab133737 showing negative staining in Glioma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)

    Immunohistochemical analysis using ab133737 showing negative staining in Breast carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)

    Immunohistochemical analysis using ab133737 showing positive staining in Lung adenocarcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)

    Immunohistochemical analysis using ab133737 showing positive staining in Thyroid gland carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)

    Immunohistochemical analysis using ab133737 showing positive staining in Thyroid gland tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)
    OI-RD Scanning - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133737).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)

    This IHC data was generated using the same anti-TTF1 antibody clone, EPR5955(2), in a different buffer formulation (cat# ab133737).

    Immunohistochemical analysis of paraffin embedded Human papillary carcinoma of thyroid gland tissue labelling TTF1 with ab133737 at 1/250 dilution.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)

    This IHC data was generated using the same anti-TTF1 antibody clone, EPR5955(2), in a different buffer formulation (cat# ab133737).

    Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling TTF1 with ab133737 at 1/250 dilution.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)
    Anti-TTF1 antibody [EPR5955(2)] - BSA and Azide free (ab227574)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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