Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22647-8] to KMT2A / MLL
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-KMT2A / MLL antibody [EPR22647-8]
    See all KMT2A / MLL primary antibodies

  • Description

    Rabbit monoclonal [EPR22647-8] to KMT2A / MLL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1 whole, KMT2A/MLL knockout HAP1 whole and HeLa lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435)
    Western blot - Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435)
    Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435) at 1/1000 dilution + HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 40 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 320 kDa
    why is the actual band size different from the predicted?



    Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 12482972) This antibody reacts with an unidentifiable protein below 315 kDa.

    Exposure time: 5.5 seconds

  • Western blot - Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435)
    Western blot - Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435)
    All lanes : Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : KMT2A/MLL knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 320 kDa why is the actual band size different from the predicted?



    Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 12482972). This antibody reacts with unidentifiable proteins below 315 kDa.  ab234435 was shown to specifically react with KMT2A/MLL in wild-type HAP1 cells as signal was lost in KMT2A/MLL knockout cells. Wild-type and KMT2A/MLL knockout samples were subjected to SDS-PAGE. ab234435 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Exposure time: 48 seconds

  • Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435)
    Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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