Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 09, 2021

Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22647-8] to KMT2A / MLL
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-KMT2A / MLL antibody [EPR22647-8]
See all KMT2A / MLL primary antibodies
Description

Rabbit monoclonal [EPR22647-8] to KMT2A / MLL
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild-type HAP1 whole, KMT2A/MLL knockout HAP1 whole and HeLa lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22647-8
Isotype

IgG
Research areas
- Epigenetics and Nuclear Signaling
- Bromodomains

Western blot - Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435)

Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435) at 1/1000 dilution + HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 40 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 320 kDa
why is the actual band size different from the predicted?

Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 12482972) This antibody reacts with an unidentifiable protein below 315 kDa.

Exposure time: 5.5 seconds
Western blot - Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435)

All lanes : Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : KMT2A/MLL knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 320 kDa why is the actual band size different from the predicted?

Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 12482972). This antibody reacts with unidentifiable proteins below 315 kDa. ab234435 was shown to specifically react with KMT2A/MLL in wild-type HAP1 cells as signal was lost in KMT2A/MLL knockout cells. Wild-type and KMT2A/MLL knockout samples were subjected to SDS-PAGE. ab234435 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument using the ECL technique.

Exposure time: 48 seconds
Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com