Anti-KMT2A / MLL antibody [BL-175-7E8] (ab243867)
![Anti-KMT2A / MLL antibody [BL-175-7E8] (ab243867)](https://www.abcam.com/ps/products/243/ab243867/Images/ab243867-334259-anti-kmt2a-mll-antibody-bl-175-7e8-immunoprecipitation.jpg "Anti-KMT2A / MLL antibody [BL-175-7E8] (ab243867)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-175-7E8] to KMT2A / MLL
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-KMT2A / MLL antibody [BL-175-7E8]
See all KMT2A / MLL primary antibodies -
Description
Rabbit monoclonal [BL-175-7E8] to KMT2A / MLL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human KMT2A/ MLL aa 720-780 (N terminal). The exact sequence is proprietary. Gene ID 4297. Found in the N-terminal 300 kDa fragment generated by proteolytic cleavage and in isoform 14P-18B of MLL1.
Database link: Q03164 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-175-7E8 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-KMT2A / MLL antibody [BL-175-7E8] (ab243867)KMT2A / MLL was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243867 at 10 µl per reaction Western blot was performed on the immunoprecipitate using ab243867 at 1/1000 dilution.
Lane 1: ab243867 IP in HEK-293T cell lysate.
Lane 2: Contol IgG in HEK-293T cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
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![Western blot - Anti-KMT2A / MLL antibody [BL-175-7E8] (ab243867)](https://www.abcam.com/ps/products/243/ab243867/Images/ab243867-334258-anti-kmt2a-mll-antibody-bl-175-7e8-western-blot.jpg)
Western blot - Anti-KMT2A / MLL antibody [BL-175-7E8] (ab243867)Western blot analysis using ab243867 at 1/1000 dilution.
Lane 1: HEK-293T whole cell lysate (50 µg).
Lane 2: HeLa whole cell lysate (50 µg).
Lane3: MCF7 whole cell lysate (50 µg).
Lane 4: HepG2 whole cell lysate (50 µg).
Lane 5: A549 whole cell lysate (50 µg).
Lane 6: SW620 whole cell lysate (50 µg).
a HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 minutes.
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![Anti-KMT2A / MLL antibody [BL-175-7E8] (ab243867)](https://www.abcam.com/ps/products/243/ab243867/Images/ab243867-3-benefits-of-recombinant-antibodies.png)
Anti-KMT2A / MLL antibody [BL-175-7E8] (ab243867)
