Anti-PREP1 antibody (ab154587)
Key features and details
- Rabbit polyclonal to PREP1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PREP1 antibody -
Description
Rabbit polyclonal to PREP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human PREP1 aa 201-436.
Database link: P55347 -
Positive control
- WB: HepG2 nuclear lysate; IP: HeLa cell lysate; ICC/IF: HeLa cell lysate;
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General notes
Keep as concentrated solution.This product was previously labelled as PKNOX1.1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PREP1 antibody (ab154587) at 1/2000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HepG2 nuclear lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
10% SDS PAGE -
HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. They were stained using ab154587 diluted at 1/1000 (green).
Nuclear staining Hoechst 33342 staining (blue). -
HeLa whole cell lysate/extract
A. 40 μg HeLa whole cell lysate/extract
B. Control with 2 μg of preimmune rabbit IgG
C. Immunoprecipitation of PREP1 protein by 2 μg of ab154587Western Blot carried out by ab154587 at a 1/1000 dilution.