Anti-KMT2A / MLL antibody [EPR22647-8] - BSA and Azide free (ab256550)
![Anti-KMT2A / MLL antibody [EPR22647-8] - BSA and Azide free (ab256550)](https://www.abcam.com/ps/products/256/ab256550/Images/ab256550-353471-anti-kmt2-MLL-antibody-epr226478-western-blot-human-hap1.jpg "Anti-KMT2A / MLL antibody [EPR22647-8] - BSA and Azide free (ab256550)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22647-8] to KMT2A / MLL - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-KMT2A / MLL antibody [EPR22647-8] - BSA and Azide free
See all KMT2A / MLL primary antibodies -
Description
Rabbit monoclonal [EPR22647-8] to KMT2A / MLL - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1 and HeLa lysates.
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General notes
Ab256550 is the carrier-free version of ab234435. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256550 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22647-8 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KMT2A / MLL antibody [EPR22647-8] - BSA and Azide free (ab256550)All lanes : Anti-KMT2A / MLL antibody [EPR22647-8] (ab234435) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : KMT2A/MLL knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 320 kDa why is the actual band size different from the predicted?Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 12482972). This antibody reacts with unidentifiable proteins below 315 kDa. ab234435 was shown to specifically react with KMT2A/MLL in wild-type HAP1 cells as signal was lost in KMT2A/MLL knockout cells. Wild-type and KMT2A/MLL knockout samples were subjected to SDS-PAGE. ab234435 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Exposure time: 48 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234435).
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![Anti-KMT2A / MLL antibody [EPR22647-8] - BSA and Azide free (ab256550)](https://www.abcam.com/ps/products/256/ab256550/Images/ab256550-3-benefits-of-recombinant-antibodies.png)
Anti-KMT2A / MLL antibody [EPR22647-8] - BSA and Azide free (ab256550)
