Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Mouse monoclonal [OTI1F5] to TLE 1
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Knockout validated
Reacts with: Human, African green monkey
Isotype: IgG2a

Product name

Anti-TLE 1 antibody [OTI1F5]
See all TLE 1 primary antibodies
Description

Mouse monoclonal [OTI1F5] to TLE 1
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment, corresponding to amino acids 180-460 of Human TLE 1 produced in E.coli (NP_005068).
Positive control
- IHC-P: Human breast adenocarcinoma, colon, kidney, kidney carcinoma, lung carcinoma, ovary, ovary carcinoma, pancreas, thyroid carcinoma, endometrium adenocarcinoma, prostate, prostate carcinoma, bladder carcinoma, lymphoma and tonsil tissues. Flow Cyt: HEK293T cells transfected with pCMV6-ENTRY TLE 1 cDNA; ICC/IF: MCF7 cells; COS7 cells transiently transfected by pCMV6-ENTRY TLE1. WB: HEK293T and HepG2 cell lysates; HEK293T cells transfected with pCMV6-ENTRY TLE 1 overexpress plasmid.
General notes

Dilute in PBS (pH7.3) before use.

The clone number has been updated from 1F5 to OTI1F5, both clone numbers name the same clone.

This product was changed from ascites to tissue culture supernatant on 6^th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purity

Protein A purified
Purification notes

ab131648 is purified from TCS by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI1F5
Isotype

IgG2a
Research areas

Western blot - Anti-TLE 1 antibody [OTI1F5] (ab131648)

All lanes : Anti-TLE 1 antibody [OTI1F5] (ab131648) at 1/500 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : TLE1 knockout HEK-293T cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 83 kDa
Observed band size: 83 kDa

Lanes 1-3: Merged signal (red and green). Green - ab131648 observed at 83 kDa. Red - loading control, ab181602 observed at 37 kDa.

ab131648 Anti-TLE 1 antibody [OTI1F5] was shown to specifically react with TLE 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265059 (knockout cell lysate ab257240) was used. Wild-type and TLE 1 knockout samples were subjected to SDS-PAGE. ab131648 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-TLE 1 antibody [OTI1F5] (ab131648)

ab131648 staining TLE1 in wild-type MCF7 cells (top panel) and TLE1 knockout MCF7 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab131648 at 1/500 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor^®488) (ab150117) at 2 µg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor^®594) (ab150080) at 2 µg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Flow Cytometry - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Flow cytometric analysis of HEK293T cells transfected with either pCMV6-ENTRY TLE1 (red) or empty vector control plasmid (blue) labelling TLE1 with ab131648 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling TLE1 with ab131648 at 1/150 dilution.
Western blot - Anti-TLE 1 antibody [OTI1F5] (ab131648)

All lanes : Anti-TLE 1 antibody [OTI1F5] (ab131648) at 1/2000 dilution

Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY TLE1 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 83 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC204037)
Immunocytochemistry/ Immunofluorescence - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunofluorescent analysis of COS7 cells transiently transfected by pCMV6-ENTRY TLE1 labelling TLE1 with ab131648 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human breast adenocarcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Carcinoma of Human kidney tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human ovary tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human ovary adenocarcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labelling TLE1 with ab131648 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [OTI1F5] (ab131648)

Immunohistochemical analysis of paraffin-embedded Human lymphoma tissue labelling TLE1 with ab131648 at 1/150 dilution.

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