Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TLE 1 antibody [TLE1/2085] - BSA and Azide free (ab237916)

Key features and details

Mouse monoclonal [TLE1/2085] to TLE 1 - BSA and Azide free
Suitable for: Protein Array, IHC-P, WB
Reacts with: Human
Isotype: IgG2a

Product name

Anti-TLE 1 antibody [TLE1/2085] - BSA and Azide free
See all TLE 1 primary antibodies
Description

Mouse monoclonal [TLE1/2085] to TLE 1 - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: Protein Array, IHC-P, WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse
Immunogen

Recombinant fragment within Human TLE 1 aa 175-338. The exact sequence is proprietary.
Database link: Q04724
Positive control
- IHC-P: Human endometrial carcinoma tissue. WB: MCF7, HepG2, SH-SY5Y and HeLa whole cell lysate.
General notes

Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Ab purified from Bioreactor Concentrate by Protein A/G.
Clonality

Monoclonal
Clone number

TLE1/2085
Isotype

IgG2a
Light chain type

kappa
Research areas

Western blot - Anti-TLE 1 antibody [TLE1/2085] - BSA and Azide free (ab237916)

All lanes : Anti-TLE 1 antibody [TLE1/2085] (ab238065) at 1 µg/ml

Lane 1 : Wild-type MCF7 cell lysate
Lane 2 : TLE1 knockout MCF7 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 83 kDa
Observed band size: 83 kDa

This data was developed using the same antibody clone in a different buffer formulation (ab238065).

This product was validated with an edited cell line that does not express the epitope recognised by this antibody. Testing with other antibodies has shown that the edited cell line produces a truncated protein.

Lanes 1 - 4: Merged signal (red and green). Green - ab238065 observed at 83 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

ab238065 was shown to react with TLE 1 in wild-type MCF7 cells in Western blot with loss of signal observed in TLE1 knockout sample. Wild-type MCF7 and TLE1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab238065 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Protein Array - Anti-TLE 1 antibody [TLE1/2085] - BSA and Azide free (ab237916)

This data was produced with ab238065, the same antibody in a different formulation with BSA and Azide.
ab238065 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western blot - Anti-TLE 1 antibody [TLE1/2085] - BSA and Azide free (ab237916)

Anti-TLE 1 antibody [TLE1/2085] - BSA and Azide free (ab237916) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 83 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE 1 antibody [TLE1/2085] - BSA and Azide free (ab237916)

Formalin-fixed, paraffin-embedded human endometrial carcinoma tissue stained for TLE 1 using ab237916 at 2 μg/mL in immunohistochemical analysis.

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