Anti-TLE 1 antibody (ab125183)
Key features and details
- Rabbit polyclonal to TLE 1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TLE 1 antibody
See all TLE 1 primary antibodies -
Description
Rabbit polyclonal to TLE 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide, corresponding to a region within amino acids 175-225 of Human TLE 1 (NP_005068.2).
-
Positive control
- Jurkat, 293T and HeLa whole cell lysates
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TLE 1 antibody (ab125183)All lanes : Anti-TLE 1 antibody (ab125183) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 83 kDa
Exposure time: 3 minutes
-
Immunoprecipitation - Anti-TLE 1 antibody (ab125183)ab125183, at 6 µg/mg lysate, detecting TLE 1 in 293T cell lysate by Immunoprecipitation.
For the subsequent Western blot, ab125183 was used at 0.4 µg/ml (1 mg of immunoprecipitate, 20% loaded in the lane).
The blot was developed using the ECL technique, with an exposure time of 30 seconds.
