Anti-Cyclin D3/CCND3 antibody (ab112034)
Key features and details
- Rabbit polyclonal to Cyclin D3/CCND3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin D3/CCND3 antibody
See all Cyclin D3/CCND3 primary antibodies -
Description
Rabbit polyclonal to Cyclin D3/CCND3 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 69% homology with Cyclin D2. Cross-reactivity with this protein has not been confirmed experimentally. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Baboon, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Saos-2 whole cell lysates. ICC/IF: formaldehyde fixed WERI cells.
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General notes
This product was previously labelled as Cyclin D3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab112034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Abcam recommends using milk as the blocking agent (1%) ICC/IF Use a concentration of 5 µg/ml. Target
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Function
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. -
Sequence similarities
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain. -
Cellular localization
Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. - Information by UniProt
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Database links
- Entrez Gene: 896 Human
- Entrez Gene: 12445 Mouse
- Entrez Gene: 100009038 Rabbit
- Entrez Gene: 25193 Rat
- Omim: 123834 Human
- SwissProt: P30281 Human
- SwissProt: P30282 Mouse
- SwissProt: P48961 Rat
see all -
Alternative names
- CCND 3 antibody
- Ccnd3 antibody
- CCND3_HUMAN antibody
see all
Images
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Western blot - Anti-Cyclin D3/CCND3 antibody (ab112034)Anti-Cyclin D3/CCND3 antibody (ab112034) at 1 µg/ml (Milk Blocking - 1%) + Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D3/CCND3 antibody (ab112034)ICC/IF image of ab112034 stained WERI cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab112034 at 5µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (3)
ab112034 has been referenced in 3 publications.
- Wang J et al. Protein inhibitor of activated STAT xa depresses cyclin D and cyclin D kinase, and contributes to the inhibition of osteosarcoma cell progression. Mol Med Rep 13:1645-52 (2016). PubMed: 26708148
- Yang P et al. Inhibition of osteosarcoma cell progression by MacroH2A via the downregulation of cyclin D and cyclin-dependent kinase genes. Mol Med Rep 11:1905-10 (2015). PubMed: 25378143
- Lei S et al. MacroH2A suppresses the proliferation of the B16 melanoma cell line. Mol Med Rep 10:1845-50 (2014). PubMed: 25119498
Images
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Western blot - Anti-Cyclin D3/CCND3 antibody (ab112034)Anti-Cyclin D3/CCND3 antibody (ab112034) at 1 µg/ml (Milk Blocking - 1%) + Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D3/CCND3 antibody (ab112034)
ICC/IF image of ab112034 stained WERI cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab112034 at 5µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
