Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cyclin D3/CCND3 antibody (ab112034)

Key features and details

  • Rabbit polyclonal to Cyclin D3/CCND3
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Cyclin D3/CCND3 antibody
    See all Cyclin D3/CCND3 primary antibodies

  • Description

    Rabbit polyclonal to Cyclin D3/CCND3

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 69% homology with Cyclin D2. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Baboon, Macaque monkey, Gorilla, Chinese hamster, Orangutan

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Saos-2 whole cell lysates. ICC/IF: formaldehyde fixed WERI cells.

  • General notes

     This product was previously labelled as Cyclin D3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab112034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Abcam recommends using milk as the blocking agent (1%)
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

  • Sequence similarities

    Belongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.

  • Cellular localization

    Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CCND 3 antibody
    • Ccnd3 antibody
    • CCND3_HUMAN antibody
    • CyclinD3 antibody
    • D3 type cyclin antibody
    • G1 S specific cyclin D3 antibody
    • G1/S specific cyclin D3 antibody
    • G1/S-specific cyclin-D3 antibody
    see all

Images

  • Western blot - Anti-Cyclin D3/CCND3 antibody (ab112034)
    Western blot - Anti-Cyclin D3/CCND3 antibody (ab112034)
    Anti-Cyclin D3/CCND3 antibody (ab112034) at 1 µg/ml (Milk Blocking - 1%) + Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa
    Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes


    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D3/CCND3 antibody (ab112034)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D3/CCND3 antibody (ab112034)

    ICC/IF image of ab112034 stained WERI cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab112034 at 5µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References (3)

Publishing research using ab112034? Please let us know so that we can cite the reference in this datasheet.

ab112034 has been referenced in 3 publications.

  • Wang J  et al. Protein inhibitor of activated STAT xa depresses cyclin D and cyclin D kinase, and contributes to the inhibition of osteosarcoma cell progression. Mol Med Rep 13:1645-52 (2016). PubMed: 26708148
  • Yang P  et al. Inhibition of osteosarcoma cell progression by MacroH2A via the downregulation of cyclin D and cyclin-dependent kinase genes. Mol Med Rep 11:1905-10 (2015). PubMed: 25378143
  • Lei S  et al. MacroH2A suppresses the proliferation of the B16 melanoma cell line. Mol Med Rep 10:1845-50 (2014). PubMed: 25119498

Images

  • Western blot - Anti-Cyclin D3/CCND3 antibody (ab112034)
    Western blot - Anti-Cyclin D3/CCND3 antibody (ab112034)
    Anti-Cyclin D3/CCND3 antibody (ab112034) at 1 µg/ml (Milk Blocking - 1%) + Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa
    Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes


    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D3/CCND3 antibody (ab112034)
    Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D3/CCND3 antibody (ab112034)

    ICC/IF image of ab112034 stained WERI cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab112034 at 5µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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