Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
![Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-14-AntiCyclin-D3CCND3-antibody-SP207-IHC-Human-placenta.jpg "Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP207] to Cyclin D3/CCND3
- Suitable for: Flow Cyt, IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Cyclin D3/CCND3 antibody [SP207]
See all Cyclin D3/CCND3 primary antibodies -
Description
Rabbit monoclonal [SP207] to Cyclin D3/CCND3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil, skin, uterus, placenta, cervix, pancreas, kidney, reactive lymph node, cervical squamous cell carcinoma, and B-cell lymphoma tissue; WB: Wild type HAP1 whole cell lysate; Flow Cyt: K562 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product was previously labelled as Cyclin D3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP207 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution (3.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-280990-anti-cyclin-d3-antibody-sp207-western-blot.jpg)
Western blot - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Empty Lane
Lane 3: CCND3 knockout HAP1 whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab183338 observed at 33 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab183338 was shown to recognize empty when empty knockout samples were used, along with additional cross-reactive bands. Wild-type and empty knockout samples were subjected to SDS-PAGE. Ab183338 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 100 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215469-anti-cyclin-d3-antibody-sp207-western-blot.jpg)
Western blot - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338) at 1/100 dilution + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 33 kDa
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![Flow Cytometry - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215470-anti-cyclin-d3-antibody-sp207-flow-cytometry.jpg)
Flow Cytometry - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Flow cytometric analysis of K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells, labeling Cyclin D3/CCND3 with ab183338 diluted 1/100 (green), compared with a negative control of anti-rabbit IgG (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215471-anti-cyclin-d3-antibody-sp207-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemical analysis of paraffin-embedded human tonsil tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215474-anti-cyclin-d3-antibody-sp207-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemical analysis of paraffin-embedded human skin tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215476-anti-cyclin-d3-antibody-sp207-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemical analysis of paraffin-embedded human uterus tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215478-anti-cyclin-d3-antibody-sp207-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemical analysis of paraffin-embedded human placenta tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215479-anti-cyclin-d3-antibody-sp207-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemical analysis of paraffin-embedded human cervix tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215480-anti-cyclin-d3-antibody-sp207-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemical analysis of paraffin-embedded human pancreas tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215481-anti-cyclin-d3-antibody-sp207-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemical analysis of paraffin-embedded human kidney tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215483-anti-cyclin-d3-antibody-sp207-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemical analysis of paraffin-embedded human reactive lymph node tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215484-anti-cyclin-d3-antibody-sp207-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-215485-anti-cyclin-d3-antibody-sp207-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue, labeling Cyclin D3/CCND3 with ab183338 at 1/100 dilution.
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![Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)](https://www.abcam.com/ps/products/183/ab183338/Images/ab183338-15-benefits-of-recombinant-antibodies.png)
Anti-Cyclin D3/CCND3 antibody [SP207] (ab183338)
