Anti-HNF-1B antibody [CL0374] (ab236759)
![Anti-HNF-1B antibody [CL0374] (ab236759)](https://www.abcam.com/ps/products/236/ab236759/Images/ab236759-313578-anti-hnf-1b-antibody-cl0374-immunohistochemistry.jpg "Anti-HNF-1B antibody [CL0374] (ab236759)")
Key features and details
- Mouse monoclonal [CL0374] to HNF-1B
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HNF-1B antibody [CL0374]
See all HNF-1B primary antibodies -
Description
Mouse monoclonal [CL0374] to HNF-1B -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human HNF-1B aa 1-150.
Database link: P35680 -
Epitope
Binds to an epitope located within the peptide sequence TPPILKELQA as determined by overlapping synthetic peptides. -
Positive control
- IHC-P: Human kidney, colon, renal cancer and colorectal cancer tissues. WB: CaCo-2 cell lysate; HNF1B Over-expressed in HEK-293T cell lysate. ICC/IF: A549 cells.
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General notes
Previously labelled as HNF1 beta.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL0374 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)
Paraffin-embedded human kidney tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.
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![Western blot - Anti-HNF-1B antibody [CL0374] (ab236759)](https://www.abcam.com/ps/products/236/ab236759/Images/ab236759-313605-anti-hnf-1b-antibody-cl0374-western-blot.jpg)
Western blot - Anti-HNF-1B antibody [CL0374] (ab236759)All lanes : Anti-HNF-1B antibody [CL0374] (ab236759) at 1 µg/ml
Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Predicted band size: 61 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (ab236759)](https://www.abcam.com/ps/products/236/ab236759/Images/ab236759-313585-anti-hnf-1b-antibody-cl0374-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (ab236759)PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling HNF-1B (green) using ab236759 at 4 µg/ml in ICC/IF.
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![Western blot - Anti-HNF-1B antibody [CL0374] (ab236759)](https://www.abcam.com/ps/products/236/ab236759/Images/ab236759-313584-anti-hnf-1b-antibody-cl0374-western-blot.jpg)
Western blot - Anti-HNF-1B antibody [CL0374] (ab236759)All lanes : Anti-HNF-1B antibody [CL0374] (ab236759) at 1 µg/ml
Lane 1 : Negative control, vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HNF-1B over-expression (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK-293T cells) cell lysate
Predicted band size: 61 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)](https://www.abcam.com/ps/products/236/ab236759/Images/ab236759-313582-anti-hnf-1b-antibody-cl0374-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)Paraffin-embedded human colorectal cancer tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)](https://www.abcam.com/ps/products/236/ab236759/Images/ab236759-313580-anti-hnf-1b-antibody-cl0374-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)Paraffin-embedded human renal cancer tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)](https://www.abcam.com/ps/products/236/ab236759/Images/ab236759-313606-anti-hnf-1b-antibody-cl0374-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)Paraffin-embedded human tonsil tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis. Negative control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)](https://www.abcam.com/ps/products/236/ab236759/Images/ab236759-313579-anti-hnf-1b-antibody-cl0374-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)Paraffin-embedded human colon tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.
