Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HNF-1B antibody [CL0374] (ab236759)

Key features and details

  • Mouse monoclonal [CL0374] to HNF-1B
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-HNF-1B antibody [CL0374]
    See all HNF-1B primary antibodies

  • Description

    Mouse monoclonal [CL0374] to HNF-1B

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human HNF-1B aa 1-150.
    Database link: P35680

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    Binds to an epitope located within the peptide sequence TPPILKELQA as determined by overlapping synthetic peptides.

  • Positive control

    • IHC-P: Human kidney, colon, renal cancer and colorectal cancer tissues. WB: CaCo-2 cell lysate; HNF1B Over-expressed in HEK-293T cell lysate. ICC/IF: A549 cells.

  • General notes

    Previously labelled as HNF1 beta. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    CL0374

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)

    Paraffin-embedded human kidney tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-HNF-1B antibody [CL0374] (ab236759)
    Western blot - Anti-HNF-1B antibody [CL0374] (ab236759)
    All lanes : Anti-HNF-1B antibody [CL0374] (ab236759) at 1 µg/ml

    Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate

    Predicted band size: 61 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (ab236759)
    Immunocytochemistry/ Immunofluorescence - Anti-HNF-1B antibody [CL0374] (ab236759)

    PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling HNF-1B (green) using ab236759 at 4 µg/ml in ICC/IF.

  • Western blot - Anti-HNF-1B antibody [CL0374] (ab236759)
    Western blot - Anti-HNF-1B antibody [CL0374] (ab236759)
    All lanes : Anti-HNF-1B antibody [CL0374] (ab236759) at 1 µg/ml

    Lane 1 : Negative control, vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : HNF-1B over-expression (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK-293T cells) cell lysate

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)

    Paraffin-embedded human colorectal cancer tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)

    Paraffin-embedded human renal cancer tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)

    Paraffin-embedded human tonsil tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis. Negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-1B antibody [CL0374] (ab236759)

    Paraffin-embedded human colon tissue stained for HNF-1B using ab236759 at 1/500 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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