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Human CD73 Antibody Pair - BSA and Azide free (ab256745)

Human CD73 Antibody Pair - BSA and Azide free (ab256745)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human CD73 Antibody Pair - BSA and Azide free
    See all CD73 kits
  • Assay type

    ELISA set
  • Range

    625 pg/ml - 40000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human CD73. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab254513), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human CD73 Capture Antibody (unconjugated) 1 x 100µg
    Human CD73 Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Cardiovascular
    • Hypoxia
    • Associated Proteins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other
  • Function

    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in disease

    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similarities

    Belongs to the 5'-nucleotidase family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P21589 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 5' NT
    • 5' nucleotidase (CD73)
    • 5' nucleotidase precursor
    • 5' nucleotidase, ecto
    • 5' nucleotidase, ecto (CD73)
    • 5'-NT
    • 5'-nucleotidase
    • 5NTD_HUMAN
    • CD73
    • CD73 antigen
    • E5NT
    • Ecto 5' nucleotidase
    • Ecto-5'-nucleotidase
    • eN
    • eNT
    • NT
    • NT5
    • NT5E
    • NTE
    • Purine 5 Prime Nucleotidase
    see all
  • Database links

    • Entrez Gene: 4907 Human
    • Omim: 129190 Human
    • SwissProt: P21589 Human
    • Unigene: 153952 Human

    Images

    • Sandwich ELISA - Human CD73 Antibody Pair - BSA and Azide free (ab256745)
      Sandwich ELISA - Human CD73 Antibody Pair - BSA and Azide free (ab256745)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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      Human CD73 ELISA Kit (ab254513)

      Applications:

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