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AM404, Anandamide transport inhibitor (ab120095)

Key features and details

  • Anandamide transport inhibitor
  • CAS Number: 198022-70-7
  • Purity: > 99%
  • Soluble in DMSO to 50 mM and in ethanol to 50 mM
  • Form / State: Solid
  • Source: Synthetic

Overview

  • Product name

    AM404, Anandamide transport inhibitor

  • Description

    Anandamide transport inhibitor

  • Biological description

    Competitive and selective anandamide transport inhibitor (IC50 = 1 μM). Active in vivo. Does not activate CB1 receptors or inhibit anandamide hydrolysis but has been shown to activate vanilloid receptors.

  • Purity

    > 99%

  • CAS Number

    198022-70-7

  • Chemical structure

    Chemical Structure

Properties

Images

  • Functional Studies - AM404, Anandamide transport inhibitor (ab120095)
    Functional Studies - AM404, Anandamide transport inhibitor (ab120095)

    SH-SY5Y cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and varied concentrations of AM404 (ab120095). Increased expression of c-Fos in SH-SY5Y cells correlates with an increase in AM404 concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab7963 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

     

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