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Anti-Bcl-2 antibody [10C4] (ab16904)

Price and availability

321 638 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Bcl-2 antibody [10C4] (ab16904)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [10C4] to Bcl-2
  • Suitable for: WB
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-Bcl-2 antibody [10C4]
    See all Bcl-2 primary antibodies
  • Description

    Mouse monoclonal [10C4] to Bcl-2
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: NIH/3T3 whole cell lysate. Mouse thymus and spleen tissue lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    10C4
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Cell survival & death
    • Cancer
    • Tumor biomarkers
    • Oncoproteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-Bcl-2 antibody [10C4] (ab16904)
    Western blot - Anti-Bcl-2 antibody [10C4] (ab16904)
    All lanes : Anti-Bcl-2 antibody [10C4] (ab16904) at 0.488 µg/ml

    Lane 1 : Mouse thymus tissue lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 3 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 26 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 secs; Lane 2,3: 180 secs.

  • Anti-Bcl-2 antibody [10C4] (ab16904)
    Anti-Bcl-2 antibody [10C4] (ab16904)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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