Human IFN gamma High Sensitivity ELISA Kit (ab46048)
Key features and details
- Sensitivity: 0.69 pg/ml
- Range: 0.78 pg/ml - 25 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Amplification Diluent Brown & blue spot 1 x 25ml 1 x 25ml Amplifier Yellow 1 x 200µl 2 x 200µl Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-IFN gamma Red 1 x 0.4ml 2 x 0.4ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 2 x 23ml IFN gamma Microplate (12 x 8 well strips) 1 unit 2 units IFN gamma Standard (Lyophilized) Yellow 2 vials 4 vials Plastic Plate Covers 2 units 4 units Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
Research areas
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IF 1
- IFG
- IFI
see all -
Database links
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
Images
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ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)
IFN gamma measured in cell culture supernatants from control or treated (48 hours; 2% PHA-M; LifeTechnologies) PBMCs, diluted 1/100-1/10000 (duplicates +/- SD).
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ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048) -
ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)IFN gamma measured in undiluted human biological fluids; mouse and rat samples (plasma and serum) were below limit of detection (duplicates +/- SD).
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ELISA: IFN gamma High Sensitivity Human ELISA Kit (ab46048)Dilution curves of IFN gamma (ab46048) and NIBSC standard (87/586). One ng of standard IFN gamma corresponds to 12.3 (+/- 1.3) IU NIBSC 87/586. Background signal subtracted (duplicates; +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab46048