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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Mitofusin 1 antibody [11E91H12] (ab126575)

Price and availability

291 484 ₸

Availability

Order now and get it on Friday March 19, 2021

Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [11E91H12] to Mitofusin 1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-Mitofusin 1 antibody [11E91H12]
    See all Mitofusin 1 primary antibodies
  • Description

    Mouse monoclonal [11E91H12] to Mitofusin 1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • H4IIE Rat cell lysate, NIH 3T3 Mouse cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Purification notes

    Produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. Near homogeneity as judged by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    11E91H12
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • GTP
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitophagy fission and fusion

Images

  • Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
    Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
    All lanes : Anti-Mitofusin 1 antibody [11E91H12] (ab126575) at 5 µg/ml

    Lane 1 : H4IIE Rat cell lysate
    Lane 2 : NIH 3T3 Mouse cell lysate
    Lane 3 : Mouse embryonic fibroblast cell line, wild-type
    Lane 4 : Mouse embryonic fibroblast cell line, Mitofusin 1-deficient
    Lane 5 : Mouse embryonic fibroblast cell line, Mitofusin 2-deficient


    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat-anti-Mouse HRP at 1/3000 dilution

    Predicted band size: 84 kDa



    Lower panel: Anti-Actin antibody (ab3280) at 0.5µg/mL.
  • Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
    Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
    All lanes : Anti-Mitofusin 1 antibody [11E91H12] (ab126575) at 5 µg/ml

    Lane 1 : H4IIE Rat cell lysate
    Lane 2 : Mouse embryonic fibroblast cell line, wild-type
    Lane 3 : Mouse embryonic fibroblast cell line, Mitofusin 1-deficient
    Lane 4 : Mouse embryonic fibroblast cell line, Mitofusin 2-deficient

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat-anti-Mouse HRP at 1/3000 dilution

    Predicted band size: 84 kDa



    Left panel: 4% milk was used as the blocking agent throughout the western blot. Right panel: 4% milk was used for the initial block and for the secondary antibody incubation, but ab126575 was diluted in Abcam’s Blocking Buffer (ab126587). Note that Anti-Mitofusin 1 antibody is not compatible with dilution in milk blocking buffer.
  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
    Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
    ab126575 at 5 µg/ml (2 hrs) staining Mitofusin 1 in paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) Mouse embryonic fibroblast cells by Immunofluorescence. Secondary: 1/2000 AlexaFluor® 488 goat anti-mouse IgG (H+L). DAPI was used to stain the cell nuclei (blue). Abcam Blocking Buffer (ab126587) was used throughout.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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