Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sonic Hedgehog antibody [5H4] (ab135240)

Key features and details

  • Mouse monoclonal [5H4] to Sonic Hedgehog
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-Sonic Hedgehog antibody [5H4]
    See all Sonic Hedgehog primary antibodies

  • Description

    Mouse monoclonal [5H4] to Sonic Hedgehog

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Purified Recombinant fragment, corresponding to amino acids 26-161 of Human Sonic Hedgehog expressed in E. Coli.

  • Positive control

    • Human Sonic Hedgehog recombinant protein; Sonic Hedgehog (amino acids 26-161)-hIgGFc transfected HEK293 cell lysate; LNCaP, HepG2, PANC1),HeLa, SK-N-SH, F9, NIH 3T3, and COS7 cell lysates; HeLa cells; Human liver cancer tissue.

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR164113-18 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [5H4] (ab135240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [5H4] (ab135240)
    Immunohistochemical analysis of Paraffin-embedded Human liver cancer tissue labelling Sonic Hedgehog with ab135240 at 1/200 dilution followed by DAB staining.
  • Western blot - Anti-Sonic Hedgehog antibody [5H4] (ab135240)
    Western blot - Anti-Sonic Hedgehog antibody [5H4] (ab135240)
    Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilution + Human Sonic Hedgehog recombinant protein

    Predicted band size: 49 kDa



    Expected MW is 41 kDa.
  • Western blot - Anti-Sonic Hedgehog antibody [5H4] (ab135240)
    Western blot - Anti-Sonic Hedgehog antibody [5H4] (ab135240)
    All lanes : Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : HEK293 cell lysate, transfected with Sonic Hedgehog (amino acids 26-161)-hIgGFc

    Predicted band size: 49 kDa

  • Western blot - Anti-Sonic Hedgehog antibody [5H4] (ab135240)
    Western blot - Anti-Sonic Hedgehog antibody [5H4] (ab135240)
    All lanes : Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilution

    Lane 1 : LNCaP cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : PANC1 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : SK-N-SH
    cell lysate
    Lane 6 : F9 cell lysate
    Lane 7 : NIH 3T3 cell lysate
    Lane 8 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

    Predicted band size: 49 kDa

  • Flow Cytometry - Anti-Sonic Hedgehog antibody [5H4] (ab135240)
    Flow Cytometry - Anti-Sonic Hedgehog antibody [5H4] (ab135240)
    Flow cytometric analysis of HeLa cells labelling Sonic Hedgehog with ab135240 at 1/200 dilution (green). Negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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