Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [8A2] to Sumo 2 + Sumo 3 - BSA and Azide free
Suitable for: IHC-P, WB, ICC
Reacts with: Mouse, Rat, Human

Product name

Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free
See all Sumo 2 + Sumo 3 primary antibodies
Description

Mouse monoclonal [8A2] to Sumo 2 + Sumo 3 - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: IHC-P, WB, ICCmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant full length protein. This information is considered to be commercially sensitive.
Positive control
- WB: HeLa, SH-SY5Y, K562, SW480, Jurkat, HEK-293T, PC-12 and NIH/3T3 whole cell lysate. Human brain, spleen and kidney tissue lysate. Rat spleen tissue lysate. Mouse brain tissue lysate. IHC-P: Mouse and rat kidney tissue. Human breast tissue. ICC: HeLa, SW480 and NIH/3T3 cells.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

ab252805 is the carrier-free version of ab81371. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab252805 is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm.

Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

8A2
Isotype

IgG2b
Research areas

Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

All lanes : Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate B276
Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 4 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 6 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 8 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)

Predicted band size: 12 kDa

This image was produced using ab81371, the same antibody clone in a different buffer.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

All lanes : Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Human kidney tissue lysate
Lane 3 : Human spleen tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Rat spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 12 kDa

This image was produced using ab81371, the same antibody clone in a different buffer.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunocytochemistry/immunofluorescence analysis of NIH/3T3 cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunocytochemistry/immunofluorescence analysis of SW480 cell line labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

This image was produced using ab81371, the same antibody clone in a different buffer.

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).
Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

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