Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [8A2] to Sumo 2 + Sumo 3 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free
    See all Sumo 2 + Sumo 3 primary antibodies

  • Description

    Mouse monoclonal [8A2] to Sumo 2 + Sumo 3 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WB, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HeLa, SH-SY5Y, K562, SW480, Jurkat, HEK-293T, PC-12 and NIH/3T3 whole cell lysate. Human brain, spleen and kidney tissue lysate. Rat spleen tissue lysate. Mouse brain tissue lysate. IHC-P: Mouse and rat kidney tissue. Human breast tissue. ICC: HeLa, SW480 and NIH/3T3 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252805 is the carrier-free version of ab81371. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab252805 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    8A2

  • Isotype

    IgG2b

  • Research areas

Images

  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
    Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
    All lanes : Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate B276
    Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 4 : SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 6 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
    Lane 8 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)

    Predicted band size: 12 kDa



    This image was produced using ab81371, the same antibody clone in a different buffer.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

    This image was produced using ab81371, the same antibody clone in a different buffer.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
    Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).

  • Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
    Western blot - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
    All lanes : Anti-Sumo 2 + Sumo 3 antibody [8A2] (ab81371) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human kidney tissue lysate
    Lane 3 : Human spleen tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 12 kDa



    This image was produced using ab81371, the same antibody clone in a different buffer.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
    Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

    This image was produced using ab81371, the same antibody clone in a different buffer.

    Immunocytochemistry/immunofluorescence analysis of NIH/3T3 cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

  • Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
    Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

    This image was produced using ab81371, the same antibody clone in a different buffer.

    Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

    This image was produced using ab81371, the same antibody clone in a different buffer.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
    Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).

  • Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
    Immunocytochemistry - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

    This image was produced using ab81371, the same antibody clone in a different buffer.

    Immunocytochemistry/immunofluorescence analysis of SW480 cell line labelling  Sumo 2 + Sumo 3 with ab81371 at 1/50 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody, ab81371 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab81371 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

    This image was produced using ab81371, the same antibody clone in a different buffer.

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Sumo 2 + Sumo 3 with ab81371 at 1/1000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). The section was incubated with ab81371 for overnight at 4℃.
    Secondary antibody only control: Ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution1).

  • Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)
    Anti-Sumo 2 + Sumo 3 antibody [8A2] - BSA and Azide free (ab252805)

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