Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free (ab247789)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR300(2)] to Sumo 2 + Sumo 3 + Sumo 4 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free
    See all Sumo 2 + Sumo 3 + Sumo 4 primary antibodies

  • Description

    Rabbit monoclonal [EPR300(2)] to Sumo 2 + Sumo 3 + Sumo 4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247789 is the carrier-free version of ab109196 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247789 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free (ab247789)
    Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free (ab247789)
    All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 12 kDa



    This data was developed using ab109196, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free (ab247789)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free (ab247789)

    This data was developed using ab109196, the same antibody clone in a different buffer formulation.

    ab109196, at 1/100, staining Sumo 2 + Sumo 3 + Sumo 4 in paraffin-embedded Human ovarian tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free (ab247789)
    Western blot - Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free (ab247789)
    All lanes : Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] (ab109196) at 1/200 dilution

    Lane 1 : Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
    Lane 2 : Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
    Lane 3 : Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
    Lane 4 : Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 12 kDa
    Observed band size: 16 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocking buffer: 5% NFDM/TBST

    This data was developed using ab109196, the same antibody clone in a different buffer formulation.
  • Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free (ab247789)
    Anti-Sumo 2 + Sumo 3 + Sumo 4 antibody [EPR300(2)] - BSA and Azide free (ab247789)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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