Complex III Immunocapture Kit (ab109800)
Key features and details
- Assay type: Quantitative
Overview
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Product name
Complex III Immunocapture Kit
See all Complex III kits -
Assay type
Quantitative -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Product overview
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex III from heart mitochondria.
ab109800 Complex III Immunocapture Kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for ab109800 include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).
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Notes
Related products
Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Properties
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Components 250 µg monoclonal Complex III antibody irreversibly cross-linked to protein G-agarose beads 1 x 250µg -
Research areas
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Relevance
Complex II (succinate-ubiquinone oxidoreductase), one of the mitochondrial respiratory chain complexes, transfers electrons from succinate generated during the citric acid cycle to Complex III (ubiquinolcytochrome c oxidoreductase), via a mobile electron shuttle, ubiquinone. Complex III transfers electrons to Complex IV (cytochrome c oxidase) via another mobile electron shuttle, cytochrome c. -
Alternative names
- coenzyme Q : cytochrome c — oxidoreductase
- cytochrome bc1 complex
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Database links
- Entrez Gene: 7384 Human
- Entrez Gene: 7385 Human
- Entrez Gene: 1537 Human
- Entrez Gene: 7386 Human
- Entrez Gene: 4519 Human
- Entrez Gene: 7381 Human
- Entrez Gene: 7388 Human
- Entrez Gene: 27089 Human
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Images
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Immunoprecipitation - Complex III Immunocapture Kit (ab109800)
Complex III immunoprecipitation using antibody ab109862/MS301 crosslinked to protein G-agarose beads as product ab109800.
