Anti-WDR3 antibody (ab176817)
Key features and details
- Rabbit polyclonal to WDR3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WDR3 antibody -
Description
Rabbit polyclonal to WDR3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human WDR3 aa 225-275. The exact sequence is proprietary. NP_006775.1.
Sequence:EIEDPEEPDPKKIKGSSPGIQDTLEAEDGAFETDEAPEDRILSCRKAGSI M
Database link: Q9UNX4 -
Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176817 was affinity purified using an epitope specific to WDR3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WDR3 antibody (ab176817)All lanes : Anti-WDR3 antibody (ab176817) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 106 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-WDR3 antibody (ab176817)Detection of WDR3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176817 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176817 was used at 0.1 µg/ml (Lane 2) represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.