Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

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Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14128(B)] to Flotillin 2/ESA
Suitable for: WB, IP
Knockout validated
Reacts with: Human

Product name

Anti-Flotillin 2/ESA antibody [EPR14128(B)]
See all Flotillin 2/ESA primary antibodies
Description

Rabbit monoclonal [EPR14128(B)] to Flotillin 2/ESA
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK293, Hela, A375 and A549 cell lysates.
General notes
Previously labelled as Flotillin 2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14128(B)
Isotype

IgG
Research areas

Western blot - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)

All lanes : Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : FLOT2 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 47 kDa
Observed band size: 47 kDa

Lanes 1-4: Merged signal (red and green). Green - ab181988 observed at 47 kDa. Red - loading control ab8245 observed at 36 kDa.

ab181988 Anti-Flotillin 2/ESA antibody [EPR14128(B)] was shown to specifically react with Flotillin 2/ESA in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266847 (knockout cell lysate ab257442) was used. Wild-type and Flotillin 2/ESA knockout samples were subjected to SDS-PAGE. ab181988 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)

Immunoprecipitation of Hela cell lysate labeling Flotillin 2/ESA using ab181988 at a 1/40 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)

All lanes : Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988) at 1/4000 dilution

Lane 1 : 293 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : A375 cell lysate
Lane 4 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa

Blocking buffer and concentration: 5% NFDM/TBST
Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988)

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