Anti-Flotillin 2/ESA antibody (ab96507)
Key features and details
- Rabbit polyclonal to Flotillin 2/ESA
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Flotillin 2/ESA antibody
See all Flotillin 2/ESA primary antibodies -
Description
Rabbit polyclonal to Flotillin 2/ESA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Flotillin 2/ESA aa 365-428 (C terminal).
Database link: Q14254 -
Positive control
- WB: HeLa. Mouse brain lysate. Rat brain lysate. IP: Human HeLa whole cell lysate. IHC-P: human lung cancer. ICC/IF: HeLa S3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flotillin 2/ESA antibody (ab96507)
Immunohistochemical analysis of paraffin-embedded human lung cancer using ab96507 at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Western blot - Anti-Flotillin 2/ESA antibody (ab96507)Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 47 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Flotillin 2/ESA antibody (ab96507)Immunofluorescence analysis of paraformaldehyde-fixed HeLa S3 cells, using ab96507 at 1:100 dilution. Image 2: Merged with DNA probe. -
Immunoprecipitation - Anti-Flotillin 2/ESA antibody (ab96507)Flotillin 2 was immunoprecipitated from 5µ of HeLa whole cell lysate with ab96507. Western blot was performed from the immunoprecipitate using ab96507. EasyBlot anti-Rabbit IgG, was used as secondary antibody.
Lane 1: HeLa lysate 5µg (Input).
Lane 2: ab96507 IP in HeLa lysate. -
Western blot - Anti-Flotillin 2/ESA antibody (ab96507)Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + Rat brain lysate at 50 µg
Predicted band size: 47 kDa10% SDS-PAGE
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Immunoprecipitation - Anti-Flotillin 2/ESA antibody (ab96507) This image is courtesy of an Abreview submitted by Prof Ritva TikkanenFlotillin 2/ESA immunoprecipitation from Human HeLa whole cell lysate using ab96507. 750 µg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.
Lane 1 : anti-Flotillin 2/ESA IP (using non-Abcam antibody)
Lane 2 : anti-Flotillin 2/ESA IP (using ab96507)
Lane 3 : Input (HeLa cell lysate)
Lane 4 : Supernatant IP (using non-Abcam antibody)
Lane 5 : Supernatant IP (using ab96507)
Western blot detection : mouse anti-Flotillin 2/ESA antibody + HRP donkey anti-mouse polyclonal secondary antibody -
Western blot - Anti-Flotillin 2/ESA antibody (ab96507)Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE
