Anti-Glucocorticoid Receptor antibody (ab3671)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human, Snake
- Isotype: IgG
Overview
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Product name
Anti-Glucocorticoid Receptor antibody
See all Glucocorticoid Receptor primary antibodies -
Description
Rabbit polyclonal to Glucocorticoid Receptor -
Host species
Rabbit -
Specificity
This antibody detects both the unactivated and activated forms of GR. -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Snake -
Immunogen
Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 150-175.
Sequence:APTEKEFPKTHSDVSSEQQHLKGQTG
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glucocorticoid Receptor antibody (ab3671)All lanes : Anti-Glucocorticoid Receptor antibody (ab3671) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) membrane enriched extract
Lane 2 : MCF7 (human breast adenocarcinoma cell line) membrane enriched extract
Lane 3 : T-47D membrane enriched extract
Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) membrane enriched extract
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) membrane enriched extract
Lane 6 : Mouse brain tissue extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunocytochemistry/Immunofluorescence analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial adenocarcinoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3671 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3671)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Thamnophis sirtalis (Common garter snake) brain tissue sections labeling Glucocorticoid Receptor with ab3671 at 1/250. Samples were blocked with 10% goat serum in 0.1M PBS. Samples were incubated with the primary antibody for 48 hours at 4°C. A biotin-conjugated goat anti-rabbit was used as the secondary antibody.
