Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] (ab228972)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22216-414] to Glucocorticoid Receptor (phospho S226)
Suitable for: WB, IHC-P, Dot blot
Reacts with: Human

Product name

Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414]
See all Glucocorticoid Receptor primary antibodies
Description

Rabbit monoclonal [EPR22216-414] to Glucocorticoid Receptor (phospho S226)
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U-2 OS treated with 1 µM Dexamethasone for 1hour whole cell lysate 20 µg (Untreated membrane), cell lysate. Dot blot: Glucocorticoid Receptor (phospho S226) peptide (aa220-231) and Glucocorticoid Receptor (phospho S226) peptide (aa225-234). IHC-P: Human endometrial cancer tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22216-414
Isotype

IgG
Research areas

Western blot - Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] (ab228972)

All lanes : Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] (ab228972) at 1/1000 dilution

Lane 1 : Untreated U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate (Untreated membrane)
Lane 2 : U-2 OS treated with 1 µM Dexamethasone for 1hour whole cell lysate 20 µg (Untreated membrane)
Lane 3 : U-2 OS treated with 1 µM Dexamethasone for 1hour whole cell lysate 20 µg (Phosphatase treated membrane

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 85 kDa
Observed band size: 86 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Glucocorticoid receptor is phosphorylated upon stimulation of dexamethasone, a corticosteroid (PMID: 28781762). Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] (ab228972)

Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Glucocorticoid Receptor (phospho S226) with ab228972 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human endometrium cancer (panel A), and no staining after alkaline phosphatase treatment (panel B) (PMID:12351702, 12225995). The section was incubated with ab228972 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Dot Blot - Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] (ab228972)

Dot blot analysis using ab228972 at 1/1000 dilution. The secondary antibody was a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Lane 1: Glucocorticoid Receptor (phospho S226) peptide (aa220-231)
Lane 2: Glucocorticoid Receptor (phospho S226) peptide (aa225-234)
Lane 3: Glucocorticoid Receptor non-phospho peptide (aa220-234)

Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes.
Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] (ab228972)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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