Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22216-414] to Glucocorticoid Receptor (phospho S226) - BSA and Azide free
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free
    See all Glucocorticoid Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR22216-414] to Glucocorticoid Receptor (phospho S226) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Dot blot: Glucocorticoid Receptor (phospho S226) peptide (aa220-231) and Glucocorticoid Receptor (phospho S226) peptide (aa225-234). IHC-P: Human endometrial cancer tissue.

  • General notes

    ab256549 is a PBS-only buffer format of ab228972. Please refer to ab228972 for recommended dilutions, protocols, and image data.

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)

    Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Glucocorticoid Receptor (phospho S226) with ab228972 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human endometrium cancer (panel A), and no staining after alkaline phosphatase treatment (panel B) (PMID:12351702, 12225995). The section was incubated with ab228972 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228972).

  • Dot Blot - Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)
    Dot Blot - Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)

    Dot blot analysis using ab228972 at 1/1000 dilution. The secondary antibody was a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

    Lane 1: Glucocorticoid Receptor (phospho S226) peptide (aa220-231)
    Lane 2: Glucocorticoid Receptor (phospho S226) peptide (aa225-234)
    Lane 3: Glucocorticoid Receptor non-phospho peptide (aa220-234)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228972).

  • Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)
    Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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