Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)
![Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)](https://www.abcam.com/ps/products/256/ab256549/Images/ab256549-1-anti-glucocorticoid-receptor-phospho-s226-antibody-epr22216-414-immunohistochemistry-endometrial-cancer-human.jpg "Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22216-414] to Glucocorticoid Receptor (phospho S226) - BSA and Azide free
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free
See all Glucocorticoid Receptor primary antibodies -
Description
Rabbit monoclonal [EPR22216-414] to Glucocorticoid Receptor (phospho S226) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Dot blot: Glucocorticoid Receptor (phospho S226) peptide (aa220-231) and Glucocorticoid Receptor (phospho S226) peptide (aa225-234). IHC-P: Human endometrial cancer tissue.
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General notes
ab256549 is a PBS-only buffer format of ab228972. Please refer to ab228972 for recommended dilutions, protocols, and image data.
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22216-414 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)
Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Glucocorticoid Receptor (phospho S226) with ab228972 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human endometrium cancer (panel A), and no staining after alkaline phosphatase treatment (panel B) (PMID:12351702, 12225995). The section was incubated with ab228972 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228972).
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![Dot Blot - Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)](https://www.abcam.com/ps/products/256/ab256549/Images/ab256549-4-anti-glucocorticoid-receptor-phospho-s226-antibody-epr22216-414-dot-blot-human.jpg)
Dot Blot - Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)Dot blot analysis using ab228972 at 1/1000 dilution. The secondary antibody was a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Lane 1: Glucocorticoid Receptor (phospho S226) peptide (aa220-231)
Lane 2: Glucocorticoid Receptor (phospho S226) peptide (aa225-234)
Lane 3: Glucocorticoid Receptor non-phospho peptide (aa220-234)Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228972).
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![Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)](https://www.abcam.com/ps/products/256/ab256549/Images/ab256549-5-benefits-of-recombinant-antibodies.png)
Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] - BSA and Azide free (ab256549)
