Anti-Glucocorticoid Receptor antibody (ab225886)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor
- Suitable for: WB, IP, IHC-P, ChIP-sequencing
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glucocorticoid Receptor antibody
See all Glucocorticoid Receptor primary antibodies -
Description
Rabbit polyclonal to Glucocorticoid Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ChIP-sequencingmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep
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Immunogen
Synthetic peptide within Human Glucocorticoid Receptor aa 150-200. The exact sequence is proprietary. (NP_000167.1).
Database link: P04150 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate (ab150035). IHC-P: Human lung carcinoma tissue. ChIPseq: Prednisolone-treated THP-1 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab225886 was affinity purified using an epitope specific to Glucocorticoid Receptor immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glucocorticoid Receptor antibody (ab225886)All lanes : Anti-Glucocorticoid Receptor antibody (ab225886) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-Glucocorticoid Receptor antibody (ab225886)Glucocorticoid Receptor was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225886 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225886 at 0.4 µg/ml.
Lane 1: ab225886 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab225886)Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Glucocorticoid Receptor using ab225886 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
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ChIP-sequencing - Anti-Glucocorticoid Receptor antibody (ab225886)Chromatin from prednisolone treated THP-1 (human monocytic leukemia cell line) cells was immunoprecipitated with ab225886 and analyzed by DNA sequencing. The figure illustrates the peak distribution of Glucocorticoid Receptor binding within a 250 Kb region of chromosome 6 as detected using ab225886.
