Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor (phospho S211)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glucocorticoid Receptor (phospho S211) antibody
See all Glucocorticoid Receptor primary antibodies -
Description
Rabbit polyclonal to Glucocorticoid Receptor (phospho S211) -
Host species
Rabbit -
Specificity
Detects endogenous levels of the Glucocorticoid Receptor only when phosphorylated at serine 211 (Mouse Ser220, Rat Ser232) -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from the human Glucocorticoid Receptor around the phosphorylation site of serine 211 (NESPPW).
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Positive control
- Human breast carcinoma tissue, extracts from HeLa cells treated with PMA
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)All lanes : Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189) at 1/500 dilution
Lane 1 : extracts from HeLa cells treated
with PMA (125ng/ml, 30min), with the immunising phosphpeptide.
Lane 2 : extracts from HeLa cells treated
with PMA (125ng/ml, 30min).
Predicted band size: 86 kDa
Observed band size: 86 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)Ab55189 at 1/50 dilution staining human breast carcinoma without (left) and with (right) immunizing phospho-peptide; paraffin embedded.
