Anti-CRF1/CRHR1 antibody (ab77686)
Key features and details
- Goat polyclonal to CRF1/CRHR1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CRF1/CRHR1 antibody
See all CRF1/CRHR1 primary antibodies -
Description
Goat polyclonal to CRF1/CRHR1 -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Human CRF1/CRHR1 aa 107-117 (internal sequence).
Sequence:C-NEEKKSKVHYH
Database link: NP_004373.2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab77686 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CRF1/CRHR1 antibody (ab77686)All lanes : Anti-CRF1/CRHR1 antibody (ab77686) at 1 µg/ml
Lane 1 : Human colon tissue lysate in RIPA buffer
Lane 2 : Human cerebellum tissue lysate in RIPA buffer
Lane 3 : Rat brain tissue lysate in RIPA buffer
Lane 4 : Mouse spinal cord tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 48 kDaIncubated with the primary antibody for 1 hour. Detected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-CRF1/CRHR1 antibody (ab77686)Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling CRF1 with ab77686 at 10µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.15% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 2μg/mL was used as the secondary antibody (green) showing cytoplasmic, and vesicle staining. Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG (10μg/mL) followed by Alexa Fluor® 488 secondary antibody (2μg/mL).
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Immunocytochemistry/ Immunofluorescence - Anti-CRF1/CRHR1 antibody (ab77686)Immunocytochemistry/immunofluorescence analysis of Neuro2a cells labelling CRF1 with ab77686 at 10µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.15% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 2ug/ml was used as the secondary antibody (green) showing plasma membrane, and vesicle staining. Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor® 488 secondary antibody (2ug/ml).
