Immunocytochemistry analysis of paraformaldehyde fixed MCF7 (human breast adenocarcinoma cell line) cells labelling CRF1/CRHR1 with ab59023 at 10 µg/mL. Cells were permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor^® 488 secondary antibody at 2 µg/mL (green). Nuclear DNA was labelled with DAPI (blue).

Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 µg/mL).