Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 02, 2021

Anti-DKC1/Dyskerin antibody [EPR10399] (ab156877)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10399] to DKC1/Dyskerin
Suitable for: WB, ICC/IF, IHC-P
Reacts with: Human

Product name

Anti-DKC1/Dyskerin antibody [EPR10399]
See all DKC1/Dyskerin primary antibodies
Description

Rabbit monoclonal [EPR10399] to DKC1/Dyskerin
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human DKC1/Dyskerin aa 350-450. The exact sequence is proprietary.
Database link: O60832
Positive control
- Recombinant Human DKC1/Dyskerin protein (ab152334) can be used as a positive control in WB. SW480 cell lysate, MCF-7 cell lysate, HeLa cell lysate, paraffin-embedded Human melanoma tissue, paraffin-embedded Human placenta tissue.
General notes
This product was previously labelled as DKC1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10399
Isotype

IgG
Research areas

Western blot - Anti-DKC1/Dyskerin antibody [EPR10399] (ab156877)

All lanes : Anti-DKC1/Dyskerin antibody [EPR10399] (ab156877) at 1/1000 dilution

Lane 1 : SW480 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF-7 cell lysate

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Predicted band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKC1/Dyskerin antibody [EPR10399] (ab156877)

Immunohistochemical analysis of paraffin embedded Human melanoma tissue labeling DKC1/Dyskerin with ab156877 antibody at a dilution of 1/50.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKC1/Dyskerin antibody [EPR10399] (ab156877)

Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling DKC1/Dyskerin with ab156877 antibody at a dilution of 1/50.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-DKC1/Dyskerin antibody [EPR10399] (ab156877)

Immunofluorescence analysis of HeLa cells labeling DKC1/Dyskerin with ab156877 antibody at a dilution of 1/100.
Anti-DKC1/Dyskerin antibody [EPR10399] (ab156877)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com