Anti-NAT2/AT-2 antibody [EPR15856] (ab194114)
![Anti-NAT2/AT-2 antibody [EPR15856] (ab194114)](https://www.abcam.com/ps/products/194/ab194114/Images/ab194114-231857-anti-nat2-antibody-epr15856-western-blot.jpg "Anti-NAT2/AT-2 antibody [EPR15856] (ab194114)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15856] to NAT2/AT-2
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
Overview
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Product name
Anti-NAT2/AT-2 antibody [EPR15856]
See all NAT2/AT-2 primary antibodies -
Description
Rabbit monoclonal [EPR15856] to NAT2/AT-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver and colon cancer lysates; HepG2 cells.
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General notes
This product was previously labelled as NAT2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15856 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NAT2/AT-2 antibody [EPR15856] (ab194114)Anti-NAT2/AT-2 antibody [EPR15856] (ab194114) at 1/5000 dilution + Human fetal liver lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-NAT2/AT-2 antibody [EPR15856] (ab194114)](https://www.abcam.com/ps/products/194/ab194114/Images/ab194114-231864-anti-nat2-antibody-epr15856-western-blot.jpg)
Western blot - Anti-NAT2/AT-2 antibody [EPR15856] (ab194114)Anti-NAT2/AT-2 antibody [EPR15856] (ab194114) at 1/1000 dilution + Human colon cancer lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-NAT2/AT-2 antibody [EPR15856] (ab194114)](https://www.abcam.com/ps/products/194/ab194114/Images/ab194114-231865-anti-nat2-antibody-epr15856-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NAT2/AT-2 antibody [EPR15856] (ab194114)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling NAT2/AT-2 with ab194114 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).
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![Anti-NAT2/AT-2 antibody [EPR15856] (ab194114)](https://www.abcam.com/ps/products/194/ab194114/Images/ab194114-4-benefits-of-recombinant-antibodies.png)
Anti-NAT2/AT-2 antibody [EPR15856] (ab194114)
