Antibodies, Proteins, Kits and Reagents for Life Science

247 929 ₸ Product size

100 µl 247 929 ₸

Order now and get it on Friday March 12, 2021

Anti-p53 (acetyl K305) antibody [EPR354(3)] (ab109396)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR354(3)] to p53 (acetyl K305)
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-p53 (acetyl K305) antibody [EPR354(3)]
See all p53 primary antibodies
Description

Rabbit monoclonal [EPR354(3)] to p53 (acetyl K305)
Host species

Rabbit
Specificity

ab109396 only detects p53 acetylated on Lysine 305.
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2 and T47-D cell lysates treated with etoposide and TSA, mouse kidney, mouse spleen and rat kidney tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR354(3)
Isotype

IgG
Research areas

Western blot - Anti-p53 (acetyl K305) antibody [EPR354(3)] (ab109396)

All lanes : Anti-p53 (acetyl K305) antibody [EPR354(3)] (ab109396) at 1/5000 dilution (purified)

Lane 1 : Untreated T47-D cell lysate
Lane 2 : T47-D treated with Etoposide and TSA

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 53 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-p53 (acetyl K305) antibody [EPR354(3)] (ab109396)

All lanes : Anti-p53 (acetyl K305) antibody [EPR354(3)] (ab109396) at 1/1000 dilution (purified)

Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-p53 (acetyl K305) antibody [EPR354(3)] (ab109396)

All lanes : Anti-p53 (acetyl K305) antibody [EPR354(3)] (ab109396) at 1/1000 dilution (unpurified)

Lane 1 : HepG2 cell lysate, untreated
Lane 2 : HepG2 cell lysate, treated with etoposide and TSA

Lysates/proteins at 10 µg per lane.

Predicted band size: 44 kDa
Anti-p53 (acetyl K305) antibody [EPR354(3)] (ab109396)

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