Anti-MIF antibody (ab34644)
Key features and details
- Chicken polyclonal to MIF
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgY
Overview
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Product name
Anti-MIF antibody
See all MIF primary antibodies -
Description
Chicken polyclonal to MIF -
Host species
Chicken -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum. -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Synthetic peptide: PMFIVNTNV PRASVPDGFL SELTQQLAQA TG conjugated to KLH, corresponding to amino acids 2-32 of Human MIF
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Positive control
- Supernatants or lysates of endotoxin-stimulated human peripheral blood mononuclear cells (PBMC).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
IgY fraction -
Purification notes
This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Western blot - Anti-MIF antibody (ab34644)All lanes : Anti-MIF antibody (ab34644) at 1/500 dilution
Lane 1 : Control
Lane 2 : 100 ng of recombinant
MIF present in a bacterial lysate
Secondary
Lane 2 : Goat anti chicken at 1/5000 dilution
Predicted band size: 14 kDa
Observed band size: 12.3 kDa why is the actual band size different from the predicted?
