Anti-IRE1 antibody (ab37073)
Key features and details
- Rabbit polyclonal to IRE1
- Suitable for: WB, ICC/IF, ICC, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IRE1 antibody
See all IRE1 primary antibodies -
Description
Rabbit polyclonal to IRE1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC MouseICC/IF MouseIHC-P RatHumanWB MouseRatHuman
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Immunogen
Synthetic peptide consisting of 16 amino acid from near the carboxy terminus (Human).
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Positive control
- WB: Rat brain tissue lysate, A20 and HeLa cell lysates. IHC-P: Rat and Human small intestine tissue. ICC: A20 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRE1 antibody (ab37073)All lanes : Anti-IRE1 antibody (ab37073) at 0.4 µg/ml
Lane 1 : Caco-2 Cell Lysate
Lane 2 : SK-N-SH Cell Lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDaDiluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody (ab37073)Immunofluorescent analysis of 4% paraformaldehyde fixed Human Small Intestine Tissue labeling IRE1 with ab37073 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Western blot - Anti-IRE1 antibody (ab37073)All lanes : Anti-IRE1 antibody (ab37073) at 0.5 µg/ml
Lane 1 : Wild-type HeLa Cell Lysate
Lane 2 : IRE1 knockout HeLa Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDaDiluting buffer and concentration: 1 hour incubation at room temperature in 5% NFDM/TBST.
Beta-actin antibody (1 μg/mL) used as loading control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody (ab37073)Immunohistochemical analysis of paraffin-embedded Rat Small Intestine Tissue using ab37073 at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Western blot - Anti-IRE1 antibody (ab37073)Western Blot of rat brain lysate labeling IRE1 with Anti-IRE1 antibody (ab37073) at (1) 0.5 and (2) 1µg/ml. -
Western blot - Anti-IRE1 antibody (ab37073)Lane 1 : Anti-IRE1 antibody (ab37073) at 0.5 µg/ml
Lane 2 : Anti-IRE1 antibody (ab37073) at 1 µg/ml
Lane 3 : Anti-IRE1 antibody (ab37073) at 2 µg/ml
All lanes : A20 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/10000 dilution
Predicted band size: 110 kDa
Observed band size: 107 kDa why is the actual band size different from the predicted?
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Immunocytochemistry - Anti-IRE1 antibody (ab37073)ab37073, at 1 µg/ml, staining IRE1 in A20 cells with by ICC.
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Immunocytochemistry/ Immunofluorescence - Anti-IRE1 antibody (ab37073)Immunocytochemistry/Immunofluorescence of IRE1p in A20 cells using ab37073 at 2 ug/ml.
