Anti-IRE1 (phospho S724) antibody [EPR23107-16] - BSA and Azide free (ab267395)
![Anti-IRE1 (phospho S724) antibody [EPR23107-16] - BSA and Azide free (ab267395)](https://www.abcam.com/ps/products/267/ab267395/Images/ab267395-383244-anti-ire1-phospho-s724-antibody-epr23107-16-bsa-and-azide-free-immunoprecipitation-hela-human.jpg "Anti-IRE1 (phospho S724) antibody [EPR23107-16] - BSA and Azide free (ab267395)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23107-16] to IRE1 (phospho S724) - BSA and Azide free
- Suitable for: WB, Dot blot, IP
- Reacts with: Human
Overview
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Product name
Anti-IRE1 (phospho S724) antibody [EPR23107-16] - BSA and Azide free
See all IRE1 primary antibodies -
Description
Rabbit monoclonal [EPR23107-16] to IRE1 (phospho S724) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa treated with 10mM DTT for 1 hour whole lysate. IP: HeLa treated with 10mM DTT for 1 hour whole lysate.
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General notes
ab267395 is the carrier-free version of ab243665. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab267395 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23107-16 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-IRE1 (phospho S724) antibody [EPR23107-16] - BSA and Azide free (ab267395)
IRE1 (phospho S724) was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10mM DTT for 1h whole cell lysate 10µg with ab243665 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243665 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: HeLa treated with 10mM DTT for 1h whole cell lysate 10µg
Lane 2: ab243665 IP in HeLa treated with 10mM DTT for 1h whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243665 in HeLa treated with 10mM DTT for 1h whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
Phosphorylation of IRE1 at S724 can be induced by DTT treatment (PMID: 31453810).
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![Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] - BSA and Azide free (ab267395)](https://www.abcam.com/ps/products/267/ab267395/Images/ab267395-1-anti-ire1phospho-s724-antibody-epr23107-16-dot-blot.jpg)
Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] - BSA and Azide free (ab267395)Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665 at 1/1,000 dilution).
Lane 1: IRE1 phospho S724 peptide.
Lane 2: IRE1 non-phospho peptide.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.
Secondary used is Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100,000 dilution.
This data was developed using the same clone but in a different formulation ab243665.
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![Anti-IRE1 (phospho S724) antibody [EPR23107-16] - BSA and Azide free (ab267395)](https://www.abcam.com/ps/products/267/ab267395/Images/ab267395-3-benefits-of-recombinant-antibodies.png)
Anti-IRE1 (phospho S724) antibody [EPR23107-16] - BSA and Azide free (ab267395)
