Anti-IRE1 antibody [9F2] (ab96481)
![Anti-IRE1 antibody [9F2] (ab96481)](https://www.abcam.com/ps/products/96/ab96481/Images/ab96481-110869-anti-ire1-antibody-9f2-western-blot.jpg "Anti-IRE1 antibody [9F2] (ab96481)")
Key features and details
- Mouse monoclonal [9F2] to IRE1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-IRE1 antibody [9F2]
See all IRE1 primary antibodies -
Description
Mouse monoclonal [9F2] to IRE1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified recombinant fragment of Human IRE1 (aa282-433) expressed in E.coli.
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Positive control
- Raji, A431, Jurkat, Hela and HEK293 cell lysate; Human brain and stomach tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
9F2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-IRE1 antibody [9F2] (ab96481)All lanes : Anti-IRE1 antibody [9F2] (ab96481) at 1/500 dilution
Lane 1 : Raji cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HEK293 cell lysate
Predicted band size: 110 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody [9F2] (ab96481)](https://www.abcam.com/ps/products/96/ab96481/Images/ab96481-110868-anti-ire1-antibody-9f2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody [9F2] (ab96481)Immunohistochemical analysis of paraffin-embedded Human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ab96481 at 1/200 dilution with DAB staining. -
![Flow Cytometry - Anti-IRE1 antibody [9F2] (ab96481)](https://www.abcam.com/ps/products/96/ab96481/Images/ab96481-110867-anti-ire1-antibody-9f2-flow-cytometry.jpg)
Flow Cytometry - Anti-IRE1 antibody [9F2] (ab96481)Overlay histogram showing Raji cells stained with ab96481 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab96481, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
