Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 103 862 ₸

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Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23107-16] to IRE1 (phospho S724)
Suitable for: WB, Dot blot, IP
Reacts with: Human

Product name

Anti-IRE1 (phospho S724) antibody [EPR23107-16]
See all IRE1 primary antibodies
Description

Rabbit monoclonal [EPR23107-16] to IRE1 (phospho S724)
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa treated with 10mM DTT for 1 hour whole lysate. IP: HeLa treated with 10mM DTT for 1 hour whole lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23107-16
Isotype

IgG
Research areas

Western blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665)

All lanes : Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665) at 1/1000 dilution

Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa treated with 10mM DTT for 1 hour whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 110 kDa
Observed band size: 110 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Phosphorylation of IRE1 at S724 can be induced by DTT treatment (PMID: 31453810).

Exposure time: 48 seconds.
Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665)

Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665 at 1/1,000 dilution).

Lane 1: IRE1 phospho S724 peptide.

Lane 2: IRE1 non-phospho peptide.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 3 mins.

Secondary used is Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100,000 dilution.
Immunoprecipitation - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665)

IRE1 (phospho S724) was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10mM DTT for 1h whole cell lysate 10µg with ab243665 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243665 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: HeLa treated with 10mM DTT for 1h whole cell lysate 10µg
Lane 2: ab243665 IP in HeLa treated with 10mM DTT for 1h whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243665 in HeLa treated with 10mM DTT for 1h whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 mins.

Phosphorylation of IRE1 at S724 can be induced by DTT treatment (PMID: 31453810).
Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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