Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5253] to IRE1 (phospho S724)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-IRE1 (phospho S724) antibody [EPR5253]
    See all IRE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5253] to IRE1 (phospho S724)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human IRE1 aa 700-800 (phospho S724). The exact sequence is proprietary.
    Database link: O75460

  • Positive control

    • WB: Thapsigargin treated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, DTT treated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.10 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5253

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)
    Western blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)
    All lanes : Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) at 1/200 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1uM Thapsigargin for 1 hour whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1uM Thapsigargin for 6 hours whole cell lysates
    Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 5 : HEK-293 (Human embryonic kidney epithelial cell) treated with 10mM DTT for 1 hour whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 110 kDa
    Observed band size: 110 kDa


    Exposure time: 180 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

  • Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)
    Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)

    Dot Blot analysis of Lane 1: IRE1 (pS724) phospho peptide and Lane 2: IRE1 non-phospho peptide labeling IRE1 (phospho S724) with ab124945 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

  • OI-RD Scanning - Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)
    OI-RD Scanning - Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)
    Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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