Anti-MELK antibody (ab273015)
Key features and details
- Rabbit polyclonal to MELK
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MELK antibody
See all MELK primary antibodies -
Description
Rabbit polyclonal to MELK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human MELK conjugated to keyhole limpet haemocyanin.
Database link: Q14680 -
Positive control
- WB: MCF7 and NIH3T3 whole cell lysate. Mouse and rat liver tissue lysate. IHC-P: Human lung cancer tissue. ICC/IF: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MELK antibody (ab273015)All lanes : Anti-MELK antibody (ab273015) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat liver tissue lysate
Predicted band size: 74 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody (ab273015)Human lung cancer tissue stained for MELK using ab273015 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody (ab273015)Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling MELK (red) using ab273015 at 1/100 dilution.