Anti-IRE1 (phospho S724) antibody [EPR5253] - BSA and Azide free (ab240009)
![Anti-IRE1 (phospho S724) antibody [EPR5253] - BSA and Azide free (ab240009)](https://www.abcam.com/ps/products/240/ab240009/Images/ab240009-2-ab124945268163ab124945Dotblot.jpg "Anti-IRE1 (phospho S724) antibody [EPR5253] - BSA and Azide free (ab240009)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5253] to IRE1 (phospho S724) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-IRE1 (phospho S724) antibody [EPR5253] - BSA and Azide free
See all IRE1 primary antibodies -
Description
Rabbit monoclonal [EPR5253] to IRE1 (phospho S724) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab240009 is the carrier-free version of ab124945 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab240009 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.10 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5253 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR5253] - BSA and Azide free (ab240009)
Dot Blot analysis of Lane 1: IRE1 (pS724) phospho peptide and Lane 2: IRE1 non-phospho peptide labeling IRE1 (phospho S724) with ab124945 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124945).
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![OI-RD Scanning - Anti-IRE1 (phospho S724) antibody [EPR5253] - BSA and Azide free (ab240009)](https://www.abcam.com/ps/products/240/ab240009/Images/ab240009-1-IRE1Primaryantibodiesab1249452.jpg)
OI-RD Scanning - Anti-IRE1 (phospho S724) antibody [EPR5253] - BSA and Azide free (ab240009)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124945).
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![Anti-IRE1 (phospho S724) antibody [EPR5253] - BSA and Azide free (ab240009)](https://www.abcam.com/ps/products/240/ab240009/Images/ab240009-3-benefits-of-recombinant-antibodies.png)
Anti-IRE1 (phospho S724) antibody [EPR5253] - BSA and Azide free (ab240009)
