Anti-CSNK2A2 antibody (ab10474)
Key features and details
- Rabbit polyclonal to CSNK2A2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CSNK2A2 antibody
See all CSNK2A2 primary antibodies -
Description
Rabbit polyclonal to CSNK2A2 -
Host species
Rabbit -
Specificity
Reacts with the alpha prime subunit. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Immunogen was a synthetic peptide, which represented a portion of human Casein kinase 2, alpha 2 polypeptide encoded within exon 11 (LocusLink ID 1459).
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Positive control
- RIPA extract from HeLa cells.
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General notes
CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis.
This product was previously labelled as CKII alpha prime polypeptide
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Primary antibody notes
CKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CSNK2A2 antibody (ab10474)
Detection of human CSNK2A1 by western blot of immunoprecipitates.
Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Antibodies: Affinity purified rabbit anti-CSNK2A1 antibody A300-199A (lot A300-199A-5) used for IP at 6 µg per reaction. CSNK2A1 was also immunoprecipitated by rabbit anti-CSNK2A1 antibody A300-198A. For blotting immunoprecipitated CSNK2A1, A300-199A was used at 1 µg/ml.
Detection: Chemiluminescence -
Western blot - Anti-CSNK2A2 antibody (ab10474)Detection of human and mouse CSNK2A1 by western blot.
Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer.
Antibody: Affinity purified rabbit anti-CSNK2A1 antibody A300-199A (lot A300-199A-5) used for WB at 0.1 µg/ml.
Detection: Chemiluminescence -
Western blot - Anti-CSNK2A2 antibody (ab10474)Detection of human CSNK2A2 by Western blot and immunoprecipitation.
A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v).
B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2 prime, or vimentin (v) siRNA.
C. RIPA extract (0.5mg) from HeLa cells.
Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 mg/0.5 mg lysate (C) for IP.
Detection of human CSNK2A2 by Western blot and immunoprecipitation. A. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2 siRNA or vimentin siRNA (v). B. RIPA extract (30 mg) from HeLa cells treated with CSNK2A2, CSNK2A2, or vimentin (v) siRNA. C. RIPA extract (0.5mg) from HeLa cells. Antibody: ab10474 used at 0.33 (A) or 0.2 (B) mg/ml for WB or 5 ug/0.5 mg lysate (C) for IP.
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Western blot - Anti-CSNK2A2 antibody (ab10474) Image courtesy of an anonymous Abreview.All lanes : Anti-CSNK2A2 antibody (ab10474) at 1/500 dilution
Lane 1 : Whole cell lysate prepared from alpha prime knock out MEFs
Lane 2 : Whole cell lysate prepared from alpha prime heterozygous MEFs
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 30 minutes
