Anti-VAPA antibody (ab225890)
Key features and details
- Rabbit polyclonal to VAPA
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-VAPA antibody
See all VAPA primary antibodies -
Description
Rabbit polyclonal to VAPA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human VAPA aa 125-175. The exact sequence is proprietary. (NP_919415.2).
Database link: Q9P0L0 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VAPA antibody (ab225890)All lanes : Anti-VAPA antibody (ab225890) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using NETN lysis buffer
Lane 2 : HeLa whole cell lysate prepared using RIPA lysis buffer
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN lysis buffer
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate prepared using NETN lysis buffer
Lane 5 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate prepared using NETN lysis buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-VAPA antibody (ab225890)VAPA was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab225890 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225890 at 1 µg/ml.
Lane 1: ab225890 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (ab225890)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for VAPA using ab225890 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
