Anti-Citrate synthetase antibody (ab151607)
Key features and details
- Rabbit polyclonal to Citrate synthetase
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Citrate synthetase antibody
See all Citrate synthetase primary antibodies -
Description
Rabbit polyclonal to Citrate synthetase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman
-
Immunogen
Synthetic peptide corresponding to Human Citrate synthetase aa 150-250 conjugated to keyhole limpet haemocyanin.
Database link: O75390 -
Positive control
- This antibody gave a positive signal in Rat Liver and Human Heart Mitochondrial tissue lysates as well as the following whole cell lysates: HepG2; Caco2; HEK293; HeLa; A375
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB RatHumanAll applications MouseHorseChickenPigChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).Target
-
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
Sequence similarities
Belongs to the citrate synthase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 100858903 Chicken
- Entrez Gene: 1431 Human
- Entrez Gene: 12974 Mouse
- Entrez Gene: 397519 Pig
- Entrez Gene: 170587 Rat
- Omim: 118950 Human
- SwissProt: P23007 Chicken
- SwissProt: O75390 Human
see all -
Alternative names
- CISY_HUMAN antibody
- Citrate synthase antibody
- Citrate synthase, mitochondrial antibody
see all
Images
-
Western blot - Anti-Citrate synthetase antibody (ab151607)All lanes : Anti-Citrate synthetase antibody (ab151607) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Human Heart Mitochondrial Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa (possible non-specific binding)
Exposure time: 30 secondsThe band observed at 48 kDa could potentially be a cleaved form of Citrate synthetase due to the presence of a 27 amino acid transit peptide.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab151607 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab151607 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-Citrate synthetase antibody (ab151607)All lanes : Anti-Citrate synthetase antibody (ab151607) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Human Heart Mitochondrial Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa (possible non-specific binding)
Exposure time: 30 secondsThe band observed at 48 kDa could potentially be a cleaved form of Citrate synthetase due to the presence of a 27 amino acid transit peptide.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab151607 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
