This assay specifically detects NAD and NADH, but not NADP nor NADPH.

If you would like to use a fluorometric reading, please refer to NAD/NADH Assay Kit (Fluorometric) (ab176723).

NAD/NADH Assay kit ab221821 uses an alternative assay method that relies on purification of NAD and NADH from samples and may be more sensitive in some samples.

Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

How other researchers have used NAD/NADH Assay Kit ab65348

This NAD/NADH assay kit has been used in publications in a variety of sample types, including:
- Human: primary blood mononuclear cells¹, epithelial ovarian cancer cells², Jurkat cells³
- Mouse: cell culture lysates⁴, cardiomyocyte cell culture lysates⁵, liver⁶, liver and muscle⁷, primary hepatocyte cell cultures⁸, aorta tissue⁹
- Rat: brain tissue¹⁰
- Locust: thoraic muscle¹¹
Bacteria: Z mobilis¹², E coli¹³

^{References: 1 - Castro-Marrero J et al 2015; 2 - Zhu J et al 2019, Xia H et al 2015; 3 - Miller TW et al 2015; 4 - Mekala NK et al 2019, Ling S et al 2017; 5 - Zhang D et al 2019; 6 - Shao D et al 2017, Mukherji A et al 2015, Yu JH et al 2016; 7 - Karandrea S et al 2017; 8 - Traboulsi H et al 2014; 9 - Liu Y et al 2016; 10 - Rao G et al 2016; 11 - Ding D et al 2018; 12 - Wu B et al 2019; 13 - Long YM et al 2017}